News

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Apr 2016
NEW PUBLICATION

Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model

Mar 2016
NEW PUBLICATION

Humanized mouse G6 anti-idiotypic monoclonal antibody has therapeutic potential against IGHV1-69 germline gene-based B-CLL

Feb 2016
NEW PUBLICATION

IGHV1-69 polymorphism modulates anti-influenza antibody repertoires, correlates with IGHV utilization shifts and varies by ethnicity.


Announcements

Access to Primary Tissue

We have IRB approval to collected tissues from renal cell carcinoma patients.
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Launch of Humanized Mouse Program

The Marasco Lab Humanized Mouse Program is open to the research community We have IRB approval to collected tissues from renal cell carcinoma patients.
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www.humouse.org

 

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Project Highlight:
3I14, an hemagglutinin (HA) stem-directed broadly neutralizing antibody, isolated from human memory B cells utilizes a heavy chain encoded by the IGHV3-30 germline gene
 

A broadly neutralizing anti-influenza antibody reveals ongoing capacity of hemagglutinin-specific memory B cells to evolve
 
  1. 3I14 binds and neutralizes group 1 and 2 influenza A viruses and protects mice from lethal challenge
  2. 3I14 overlaps with or very close to the known stem epitopes of other broadly neutralizing antibodies
  3. 3I14 binding to the HA stem epitope leads to inhibition of HA0 cleavage and pH-dependent conformational changes
  4. Similar to other anti-stem broadly neutralizing antibodies, 3I14 engages an Fc-dependent immune-mediated mechanism
  5. Memory B cell evolution can expand the HA subtype specificity; evidence further suggests that establishing an optimized memory B cell pool should be an aim of "universal" influenza vaccine strategies

Superimposition of 3I14 model with FI6v3, d9.29 and MAb3.1. The heavy chain is in the blue, the light chain is in cyan. The HCDR3s and LCDR1s are indicated by the ovals. The residues in HCDR3 of 3I14, FI6v3, 39.29 and MAb 3.1 are colored in red, magenta, yellow and green, respectively.